Journal: Acta pharmaceutica Sinica. B

Article Title: Chlorella sp.-ameliorated undesirable microenvironment promotes diabetic wound healing.

doi: 10.1016/j.apsb.2022.06.012

Figure Lengend Snippet: Figure 1 Chlorella axenic culture and characterization of Chlorella hydrogels. (A) Axenic validation of Chlorella with BG-11 agar plate detected by an inverted fluorescence microscope. Scale bars: 500 mm. (B) Axenic validation of Chlorella with beef extract peptone agar plates detected by colony count. (C) Re-suspending stability of Chlorella in BG-11 medium for 12 h standing, 37 C, 800 lx. (D) Cytotoxicity of Chlorella (1 109 cell/mL) on L-929, HaCaT and HUVEC cells for 24 h (n Z 6). (E) SEM photographs of Chlorella hydrogels. Scale bars: 100 mm in 200 , 10 mm in 2000 . (F) Photographs of Chlorella hydrogels after preparation and Day 30 after storage. (G) Proliferation of axenic Chlorella in BG-11 medium and the hydrogels with BG-11 for 24 h, 37 C, 800 lx, respectively (n Z 6). The concentrations of axenic Chlorella: 1 106, 1 107, 1 108 and 1 109 cell/mL. (H) Proliferation of axenic Chlorella in the hydrogels after storage for 30 days, 4 C, dark (n Z 6). (I) Residual NO3 e in axenic Chlorella hydrogels after storage for 30 days, 4 C, dark (n Z 6). Data are presented as mean SD.

Article Snippet: After the colonies formation, the Chlorella was photographed by an inverted fluorescence microscope (IX53, Nikon, Japan).

Techniques: Biomarker Discovery, Microscopy

Journal: Journal of Nanobiotechnology

Article Title: A novel perspective on the regulation of cardiac cell beating: cardiac cell under mechanical stimulation acts as “cell activation button” to activate adjacent cardiac cell

doi: 10.1186/s12951-025-03244-x

Figure Lengend Snippet: The micro-manipulation platform for mechanical stimulation of cardiac cells. The whole experiment is performed on this platform. ( a ) and ( b ) are the physical diagram and schematic diagram of the micro-manipulation platform respectively. The platform mainly consists of an inverted microscope with an X-Y electric stage, a micromanipulator and a camera. Through the real-time feedback captured by the camera, the stage is controlled to move cardiac cells to the target position, and the 3-degree-of-freedom micromanipulator loaded with a mechanical probe is controlled to apply the mechanical stimulation of the cardiac cells

Article Snippet: This platform consists of an inverted optical microscope (Olympus, IX − 53); An electric X-Y stage (Proscan III, prior) with a motion range of 120-mm×80-mm and a positioning resolution of 0.04-μm.

Techniques: Inverted Microscopy